PE-labeled Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer Protein,His-Avi Tag性能參數(shù)表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)PE-labeledHumanHLA-A*02:01&B2M&P53R175H(HMTEVVRHC)TetramerProteinisexpressedfromHEK293withHistagandAvitagattheC-Terminus.PE-labeledHumanHLA-A*02:01&B2M&P53R175H(HMTEVVRHC)TetramerisassembledbybiotinylatedmonomerandPE-labeledstreptavidin.ItcontainsGly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)andHMTEVVRHCpeptide.(Endotoxin)Lessthan1EUperμgbytheLALmethod.制劑(Formulation)Suppliedas0.22μmfilteredsolutionin20mMPB,500mMNaCl,0.2%BSA(pH8.0).儲(chǔ)存條件Theproductshouldbestoredat-85~-65℃for3monthfromdateofreceipt.Recommendtoaliquottheproteinintosmallerquantitieswhenfirstusedandavoidrepeatedfreeze-thawcycles.α-凝血酶(α-Thrombin)是一種高度特異性的絲氨酸蛋白酶,由凝血酶原經(jīng)蛋白水解活化而來(lái)。Recombinant Canine ROR1 Protein,His Tag
Recombinant Biotinylated Human KIR2DL1 Protein,His-Avi Tag性能參數(shù)分子別名(Synonyms)CD158A;CD158Ankat1;cl-42表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)BiotinylatedHumanKIR2DL1ProteinisexpressedfromHEK293withHistagandAvitagattheC-Terminus.ItcontainsHis22-Arg242.[Accession|P43626]分子量大小(MolecularWeight)TheproteinhasapredictedMWof27.1kDa.Duetoglycosylation,theproteinmigratesto48-60kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重構(gòu)方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.Recombinant Human SCF Protein重組型TEV蛋白酶(rTEV)是經(jīng)過(guò)基因工程改造和純化后的重組蛋白酶,保持天然TEV酶的功能活性。
Coronavirusesarecommonlycomprisedoffourstructuralproteins:Spikeprotein(S),Envelopeprotein(E),Membraneprotein(M)andNucleocapsidprotein(N).TheSARS-CoV-2Sproteinisaglycoproteinthatmediatesmembranefusionandviralentry.TheSproteinishomotrimeric,witheach~180-kDamonomerconsistingoftwosubunits,S1andS2.TheRBDofSARS-CoV-2bindsametallopeptidase,angiotensin-convertingenzyme2(ACE-2).產(chǎn)品性質(zhì)別名SproteinRBD;SglycoproteinRBD;SpikeproteinRBDAccessionQHD43416.1表達(dá)區(qū)間及表達(dá)系統(tǒng)RecombinantSARS-CoV-2SpikeRBD(OmicronBA.4/BA.5/BA.5.1.3/BA.5.2)ProteinisexpressedfromExpi293withHistagattheC-terminal.ItcontainsArg319-Phe541(G339D,S371F,S373P,S375F,T376A,D405N,R408S,K417N,N440K,L452R,S477N,T478K,E484A,F486V,Q498R,N501Y,Y505H).
α-凝血酶(α-Thrombin)產(chǎn)品性質(zhì)中文別名(Chinesesynonym)α-凝血酶;IIa因子;英文別名(Englishsynonym)α-Thrombin;FactorIIaCAS號(hào)(CASNO.)9002-04-4分子量(Molecularweight)37000daltons活力(Activity)≥3091.00NIHU/mg緩沖液組分(Buffer)50mMSodiumCitrate/0.2MNaCl/0.1%PEG-8000/pH6.5含量(Totalprotein)0.324mg運(yùn)輸和保存方法粉末冰袋運(yùn)輸,2-8℃保存;配好的液體,請(qǐng)于≤-60℃保存。使用方法(酶切體系)產(chǎn)品溶于水,配成的1000U/ml儲(chǔ)存液,根據(jù)使用量分配于不同的離心管中,凍存于?20℃保存,凝血酶對(duì)不同的融合蛋白切割效率是不一樣的,首先要進(jìn)行小量切割試驗(yàn)。比如固定融合蛋白10ug,加不同量的凝血酶(如0.1U,0.2U,0.5U,1U等),在不同的溫度(如4度,16度,室溫或37度等)下進(jìn)行試驗(yàn)。FGF-18與FGF R2C,F(xiàn)GF R3C以及高爾基蛋白GLG1結(jié)合,并誘導(dǎo)星形膠質(zhì)細(xì)胞和小膠質(zhì)細(xì)胞。
Cas9核酸酶是一種引導(dǎo)RNA引導(dǎo)的核酸內(nèi)切酶,可以催化雙鏈DNA的裂解。這種靶向核酸酶是一種高精度的基因組編輯的有力工具。Cas9蛋白與CRISPR/Cas9系統(tǒng)的引導(dǎo)RNA(gRNA)成分形成一個(gè)非常穩(wěn)定的核糖白(RNP)復(fù)合物。Cas9RNP復(fù)合物可以在進(jìn)入細(xì)胞后,通過(guò)添加一個(gè)N端核定位信號(hào)(NLS),增加入核效率。YEASEN開(kāi)發(fā)的NLS-Cas9核酸酶在蛋白的N端包含一個(gè)核定位序列(NLS),以增加入核切割效率。產(chǎn)品特點(diǎn)如下:無(wú)DNA:沒(méi)有外部DNA添加。安全性好:野生型Cas9蛋白,無(wú)標(biāo)簽??蓱?yīng)用于:通過(guò)體外DNA切割篩選高效和特異性靶向gRNA。產(chǎn)品信息貨號(hào)11366ES60/11366ES76規(guī)格100μg/500μg來(lái)源重組Cas9來(lái)源于大腸桿菌物種化膿性鏈球菌標(biāo)簽無(wú)分子量160KDa濃度10mg/mL(50μg);10mg/mL(100μg)凝血酶是由大小分別為31 KD和6 KD的兩條肽鏈通過(guò)二硫鍵組成的一種絲氨酸蛋白水解酶。Recombinant Mouse LCAT Protein,His Tag
26Rfa, Hypothalamic Peptide, human,純度:經(jīng)SDS-PAGE和HPLC鑒定純度大于98%。Recombinant Canine ROR1 Protein,His Tag
透明質(zhì)酸酶活力單位(Unitdefinition)基于USP透明質(zhì)酸酶參照標(biāo)準(zhǔn),即每分鐘在37℃,pH5.7的2mL反應(yīng)液中引起OD6000.330個(gè)變化定義為一個(gè)活力單位。抑制劑(Inhibitor)Fe2+,F(xiàn)e3+,Zn2+,Cu2+,肝素,金精三羧酸,硫酸,硝酸,乙?;该髻|(zhì)酸,硫酸纖維素酯,膽汁等結(jié)構(gòu)式(Structure)運(yùn)輸和保存方法冰袋運(yùn)輸。-20℃保存,兩年有效。溶液配制溶于0.02MPBS(pH7.0),含77mMNaCl和0.01%BSA,濃度為1mg/mL?,F(xiàn)配現(xiàn)用。該產(chǎn)品儲(chǔ)存液不穩(wěn)定,使用時(shí)建議現(xiàn)配現(xiàn)用。為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。Recombinant Canine ROR1 Protein,His Tag