牛纖維蛋白原不僅在血液凝固和傷口愈合中發(fā)揮著重要作用,而且在生物醫(yī)學研究中具有廣泛的應用前景。深入研究其分子特性和生物學功能,有助于開發(fā)新的策略,以應對與血液凝固相關(guān)的疾病。未來方向未來的研究可以集中在以下幾個方面:牛纖維蛋白原與凝血級聯(lián)反應中其他組分的相互作用。牛纖維蛋白原在不同疾病狀態(tài)下的功能變化。基于牛纖維蛋白原的新型生物材料的開發(fā)。本綜述總結(jié)了牛纖維蛋白原的分子特性和生物醫(yī)學應用,為未來的研究提供了方向,并強調(diào)了其在疾病和生物材料開發(fā)中的潛力。透明質(zhì)酸的生產(chǎn)可以通過動物組織提取、微生物發(fā)酵或化學合成等方法進行。Recombinant Canine TAG-72 Protein,His Tag
AngiotensinIConvertingEnzyme(ACE-2),alsocalledACEH(ACEhomologue),isadimeric,zinc-dependentmetalloproteaseoftheACEfamilythatalsoincludessomaticandgerminalACE.ACE-2mRNAisfoundathighlevelsinheart,testis,andkidneyandatlowerlevelsinawidevarietyoftissues.ACE-2istheSARS-CoVandSARS-CoV2Spikeproteinreceptorinvivo,functionscatalyticallyasacarboxypeptidasetocleaveseveralsubstratesincludingangiotensinsIandII,andactsasapartnerforB0AT1-familyaminoacidtransporters.Throughthesefunctions,ACE-2hasbeenshowntobeinvolvedinseveraldiseasesincludingSARS,COVID19,acutelunginjury,heartdisease,liverandlungfibrosis,inflammatorylungdisease,andcardiopulmonarydisease.FulllengthACE-2proteinincludesanextracellularregioncomposedofasingleN-terminalpeptidasedomainandC-terminalcollectrin-likedomain(CLD),atransmembranedomain,andashortcytoplasmictail.TheN-terminalpeptidaseregionisrequiredforbindingtoSARS-CoVandSARS-CoV2spikeproteins,whiletheCLDcontainsaregionthatpromotesdimerizationandassociationwithaminoacidtransporters.Recombinant Human Siglec-5/CD170 Protein,His-Avi Tag跨膜蛋白的表達與制備需要采用合適的表達載體、宿主細胞、培養(yǎng)條件和純化工藝等方法,優(yōu)化表達和純化過程。
糖苷酶F(PNGaseF)酶活定義(UnitDefinition)1個酶活力單位指在10μL的反應體系中,37℃條件下1小時從10μg變性RNaseB中除去超過95%的碳水化合物所需要的酶量。儲存條件-25~-15℃保存,有效期1年。使用方法1、變性條件下蛋白質(zhì)去糖基化:1)在水中加入1μLBuffer1和目標糖蛋白(1-20μg),至終體積10μL;2)100℃溫度下煮沸10min使其變性,冰上冷卻,離心10秒;加入2μL的Buffer2、2μL的10%NP-40、6μL去離子水,總反應體積20μL;加入1-2μL的PNGase,輕輕混勻。在37℃孵育1-3h。2、非變性條件下蛋白質(zhì)去糖基化:1)在水中加入2μL的Buffer2和目標糖蛋白(1-20μg)至體積為20μL。2)加入2~5μL的PNGaseF,輕輕混勻。3)37°C孵育4-24h。注意:在變性條件下大多數(shù)底物能夠更好的去糖基化,在非變性條件下可能需要增加PNGaseF的量和延長孵育時間。注意事項1.PNGaseF建議搭配我司提供的配套緩沖液使用,按我司說明書建議的操作量配套緩沖液足夠。如您由于使用體系造成配套緩沖液不足,可咨詢當?shù)劁N售進行購買
Trop-2,alsoknownasepithelialglycoprotein-1antigen(EGP-1),isaproteinthatinhumansisencodedbytheTACSTD2gene.Mutationsofthisgeneresultingelatinousdrop-likecornealdystrophy,anautosomalrecessivedisordercharacterizedbyseverecornealamyloidosisleadingtoblindness.產(chǎn)品性質(zhì)別名EGP1;EGP-1;TROP2;GA733-1;gp50;T16;TACSTD2;TROP-2;M1S1;TACD2UniprotNo.P09758表達區(qū)間及表達系統(tǒng)RecombinantBiotinylatedHumanTROP-2/TACSTD2ProteinisexpressedfromHEK293cellswithHistagandAvitagattheC-terminal.ItcontainsHis27-Thr274.分子量TheproteinhasapredictedMWof30.5kDa.Duetoglycosylation,theproteinmigratesto46-55kDabasedonTris-BisPAGEresult.純度>95%asdeterminedbySDS-PAGEandHPLC活性ELISAData:ImmobilizedAnti-TROP-2Antibody,hFcTagat0.5μg/mL(100μL/well)ontheplate.DoseresponsecurveforBiotinylatedHumanTROP-2,HisTagwiththeEC50of24.1ng/mLdeterminedbyELISA.0EUper1μgoftheproteinbytheLALmethod.制劑Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally5%trehaloseisaddedasprotectantbeforelyophilization.在藥物篩選中,全長CB1受體可用于高通量篩選實驗,以發(fā)現(xiàn)和優(yōu)化潛在的藥物候選物。
RecombinantBiotinylatedHumanHBV(HLA-A*02:01)Protein,His-AviTag性能參數(shù)表達區(qū)間及表達系統(tǒng)(Source)RecombinantBiotinylatedHumanHBV(HLA-A*02:01)ProteinisexpressedfromHEK293withHistagandAvitagattheC-terminalItcontainsGly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)andFLLTRILTIpeptide.[Accession|A0A140T913(HLA-A*02:01)&P61769(B2M)&FLLTRILTIpeptide]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof50.5kDa.Duetoglycosylation,theproteinmigratesto52-62kDabasedonTris-BisPAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedby>95%asdeterminedbyHPLC制劑(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重構(gòu)方法(Reconstitution)Centrifugetubesbeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.C5AR與其配體C5a結(jié)合后,可以激發(fā)多種免疫細胞,促進炎癥反應和細胞趨化。Recombinant Mouse Beta Klotho Protein,His Tag
CB1受體包含多個跨膜α-螺旋結(jié)構(gòu)域,這些結(jié)構(gòu)域使得受體能夠嵌入細胞膜中。 它具有七個跨膜區(qū)域。Recombinant Canine TAG-72 Protein,His Tag
RecombinantBiotinylatedHumanHLA-A*03:01&B2M&KRASG12V(VVGAVGVGK)MonomerProtein,His-AviTag性能參數(shù)分子別名(Synonyms)MHC;KRAS;K-Ras2;KRAS2;C-K-RAS;CFC2;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;KRAS1;KRAS2;NS;NS3;RASK2;GTPaseKras;KI-RAS;RALD表達區(qū)間及表達系統(tǒng)(Source)BiotinylatedHumanHLA-A*03:01&B2M&KRASG12V(VVGAVGVGK)MonomerProteinisexpressedfromHEK293withHistagandAvitagattheC-TerminusItcontainsGly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M)andVVGAVGVGKpeptide.[Accession|NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVGAVGVGK]分子量大?。∕olecularWeight)TheproteinhasapredictedMWof50.09kDa.Duetoglycosylation,theproteinmigratesto51-60kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.純度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制劑(Formulation)Suppliedas0.22μmfilteredsolutioninPBS(pH7.4).Recombinant Canine TAG-72 Protein,His Tag